Thin frozen film method for visualization of storage proteins in mature rice grains.

Yuhi Saito,Nobuaki Nakatsuka,Takanari Shigemitsu,Kunisuke Tanaka,Shigeto Morita,Shigeru Satoh,Takehiro Masumura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY（2014）

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摘要

There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.

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关键词

immunofluorescence microscopy,thin fresh-frozen section,rice grain,storage protein,prolamin

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