The influence of β-elemene on expression of eIF4E in non-small cell lung cancer A549 cells

Objective To investigate the influence and mechanism of β-elemene on expression of eIF4E in non-small cell lung cancer A549 cells.Methods After cells treated with 20 μg/mL,40 μg/mL,60 μg/mL,80 μg/mL,100 μg/mL and 120 μg/mL β-elemene and cisplatin,Western blot was applied to detect protein expression of eIF4E;MTT was applied to observe the inhibition of β-elemene to lung cancer cell in vitro;TUNEL was used to test the apoptosis.Results eIF4E protein expression was significantly reduced in 24h and 48h.MTT and TUNEL showed that the inhibition rate and apoptotic rate of β-elemene on A549 cells indicated a dose-dependent property.Conclusion The proliferation of A549 cell was inhibited obviously by β-elemene and the mechanism may be related with down-regulation of eIF4E expression.