Production of maltooligosaccharides from starch and separation of maltopentaose by adsorption of them on activated carbon (I)
Biotechnology and Bioprocess Engineering(1997)
摘要
For the selective production of maltopentaose (G 5 ) over other oligosaccharides, enzymatic hydrolysis conditions of starch by commercial α-amylase (Termamyl ® ) were investigated. The determined optimum condition was 29.6 KNU (Kilo Novo α-amylase Unit) enzyme loading in 150 mL of 0.3% starch solution under pH level of 5 at 40°C for 30 min. About 40% of G 5 selectivity can be attained using the determined optimum condition. For further enhancing G 5 selectivity, an activated carbon adsorption process has been attached after the enzymatic hydrolysis. From the adsorption process, G 5 can be enriched up to 72% in the solution.
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关键词
maltooligosaccharides,maltopentaose,α-amylase,starch,activated carbon
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