Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs.
Journal of Insect Physiology(2010)
摘要
In an effort to understand whether heat shock protein 70 (Hsp70) participates in the environmental 5°C signal reception/transduction toward breaking embryonic diapause of the silkworm Bombyx mori, we isolated a cDNA for Hsp70a and examined the expression of Hsp70a mRNA in B. mori diapause and nondiapause eggs by quantitative real-time PCR. Hsp70a mRNA gradually increased in diapause eggs continuously kept at 25°C after oviposition to maintain diapause. When diapause eggs were exposed to the diapause-terminating condition of 5°C beginning at 2 days post-oviposition, Hsp70a mRNA increased beginning at 5 days post-cold treatment. Even in nondiapause eggs, Hsp70a mRNA increased slightly with exposure to 5°C. These results suggest that Hsp70a is involved in reception/transduction of the diapause-terminating (5°C) signal via gene activation. The expression patterns of Hsp70a mRNA are discussed in relation to those of the cold-response gene Samui.
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关键词
Embryonic diapause,Environmental cold-signal,Hsp70a,Samui,Bombyx mori
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