Quantification of bacterial phagocytosis by flow cytometry and spectrofluorimetry

    Journal of immunological methods, Volume 174, Issue 1-2, 1994, Pages 195-202.

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    macrophagehanks' balanced salt solutionfluorescein isothiocyanatemlbacterial phagocytosisMore(6+)

    Abstract:

    Bacterial phagocytosis is a cardinal function of phagocytes. We describe a simple procedure to easily quantify this function using fluoresceinated bacteria. Non-ingested bacteria and those adsorbed to the cell membrane are eliminated by an enzymatic procedure. Only macrophages with ingested fluorescent bacteria are detected, thereby permi...More

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