Direct and rapid electrochemical immunosensing system based on a conducting polymer.

A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen–antibody interaction model using ovalbumin–anti-ovalbumin. It was shown that the system specifically detects via electrochemical signal the antigen–antibody immune interaction in a reagentless context. Comparison to the conventional ELISA technique relevant to performance and sensitivity was presented.