Cloning and Transcriptional Expression Analysis of ARE Gene in Rhodosporidium toruloides

The ARE gene of Rhodosporidium toruloides was cloned and its transcriptional expression level analyzed. 2 624 bp DNA sequence of ARE gene with 6 introns was clarified. The open reading frame was 2 028 bp coding 675 amino acids. The transcriptional expression of ARE gene in the cells cultivated in nitrogen-limited medium increased with culture time. It was supposed that the transcriptional expression of ARE gene was related to lipid accumulation in R. toruloides.