Identification And Characterization Of A Novel Allergen From Blomia Tropicalis: Blo T 21
The Journal of allergy and clinical immunology(2007)
摘要
Background: Allergenic components from Blomia tropicalis are important triggers of allergies in the tropics.Objective: We sought to identify and characterize a novel allergen, Bin t 21, from B tropicalis.Methods: Blo t 21 was initially identified from an expressed sequence tag database generated from a B tropicalis cDNA library. Allergenicity of this antigen was examined by means of skin prick testing, ELISA, and IgE immuno-dot blotting. We evaluated whether Blo t 21 and Bin t 5 were cross-reactive by using IgE inhibition ELISAs.Results: Blo t 21, a 129-amino-acid protein sharing 39% identity with Blo t 5, is a product of a single-copy gene. It has an oz-helical secondary structure and localizes to midgut and hindgut contents of B tropicalis, as well as fecal particles. Positive responses to Blo t 21 were shown in 93 % (40/43) by means of ELISA and 95 % (41/43) by means of skin prick testing when assayed in 43 adult patients with ongoing persistent allergic rhinitis. However, sera of 494 consecutive individuals attending outpatient allergy clinics over 1 1/2 years showed 57.9% (286/494) had positive responses to Blo t 21. Although the majority (>75%) of sensitized individuals were cosensitized to both Blo t 5 and Blo t 21, these 2 allergens had a low-to-moderate degree of cross- reactivity.Conclusion: Blo t 21 is a major allergen in B tropicalis that is not highly cross-reactive to Blo t 5, despite sharing some sequence and structural identity. Clinical implications: Blo t 21, representing a new group of allergens, is an important B tropicalis allergen.
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关键词
dust mite,Blomia tropicalis,allergen,IgE binding,cross-reactivity,immunolocalization,genomic organization
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