CD45 isoform expression is associated with different susceptibilities of human naive and effector CD4+ T cells to respond to IL-4.

Interleukin-4 (IL-4) is the major factor promoting the development of T helper type 2 (Th2) cells from naive precursor T cells. Minute amounts of IL-4 produced by naive T cells seem to be sufficient; however, the molecular mechanisms explaining this efficient utilization of IL-4 are not yet known. Here, we show that human CD4(+) CD45RA(+) naive T cells, in contrast to CD4(+) CD45RO(+) effector T cells, show responsiveness to endogenous as well as exogenous IL-4 to proliferate and differentiate towards Th2 cells in vitro. Despite production levels of IL-4 below conventional detection limits, CD45RA(+) T cell-derived IL-4 could clearly activate STAT6. Although the expression levels of IL-4R and STAT6 were not different between naive and effector T cells, only naive T cells responded to IL-4 in a STAT6-dependent reporter gene assay. Transfecting a trans-dominant negative form of STAT6 abrogated IL-4-induced proliferation in CD45RA(+) cells. A significantly higher protein tyrosine phosphatase (PTPase) activity was detected in CD45RO(+) T cells as compared to CD45RA(+) T cells. Cross-linking CD45 potently reduced PTPase activity in CD45RO(+) T cells and restored their ability to proliferate in response to IL-4. Thus, CD45 PTPase activity contributes to T lymphocyte the susceptibility of naive and memory T cells to respond to IL-4.