Po-660-06 atrial-like engineered cardiac tissue as a novel in vitro cardiac model

Animal atrial disease models are inadequate representations of human pathophysiology and human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CM) in monolayer (ML) poorly model adult human myocardium, especially for long-term studies. To address this, we present a three-dimensional (3D) atrial-like engineered cardiac tissue (ECT) composed of atrial-like hiPSC-CM and hiPSC-cardiac fibroblasts co-cultured in a fibrin matrix that can be used to model chronic effects of atrial disease.