Electrospray ionization mass spectrometry analysis of the apo- and metal-substituted forms of the Fur protein.
FEBS Letters(1997)
摘要
Fur has been purified and reconstituted with Co2+ and Mn2+. The ESI-MS spectra of the apoprotein as well as Mn-Fur and Co-Fur under acidic denaturating conditions showed the existence of two species of molecular mass 16 660±3 and 16 792±3 Da, which correspond, respectively, to the N-terminal methionine `excised' or `non-excised' forms of the monomer. This result proves the absence of any other post-translational modification or modification due to metal incorporation. On the other hand, under soft conditions, ESI spectra provided for the first time direct evidence for dimeric metal-containing forms in solution.
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关键词
Ferric uptake regulation (Fur) protein,Iron uptake regulation,Electrospray ionization mass spectrometry,Escherichia coli
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