Effect of Na+, K+, Mg2+, Ca2+ and glucose solutions as insemination-activation media on the fertilization of Acipenser sinensis milt.

The fertilization of Chinese Sturgeon (Acipenser sinensis) milt was assessed by using different concentrations of NaCl,KCl,MgCl_2,CaCl_2 or glucose solution as the insemination-activation medium.The results showed that the fertilization rates of Chinese Sturgeon were all improved when suitable concentrations of NaCl,KCl,MgCl_2,CaCl_2 and glucose solutions were used,and that the fertilization rates reached the highest when the following concentrations of solutions were used: 87.72% in 25 mmol/L NaCl,86.82% in 0.1 mmol/L KCl, 82.24% in 1 mmol/L MgCl_2, 89.76% in 1 mmol/L CaCl_2, and 80.92% in 50 mmol/L glucose.Further increase in the salt or glucose concentration caused gradual decrease in fertilization rate. The results indicate that,as insemination-activation medium of Chinese Sturgeon milt, the most suitable concentration for NaCl, KCl, MgCl_2, CaCl_2 and glucose solution were 25 mmol/L, 0.1 mmol/L, 1 mmol/L, 1 mmol/L, and 50 mmol/L, respectively.