Identification Of Thioredoxin Target Disulfides In Proteins Released From Barley Aleurone Layers
Journal of proteomics(2010)
摘要
Thioredoxins are ubiquitous disulfide reductases involved in a wide range of cellular processes including DNA synthesis, oxidative stress response and apoptosis. In cereal seeds thioredoxins are proposed to facilitate the germination process by reducing disulfide bonds in storage proteins and other targets in the starchy endosperm. Here we have applied a thiol-specific labeling approach to identify specific disulfide targets of barley thioredoxin in proteins released from barley aleurone layers incubated in buffer containing gibberellic acid. (C) 2010 Elsevier B.V. All rights reserved.
更多查看译文
关键词
Thioredoxin,Disulfide,Redox proteomics,Isotope-coded affinity tag,Barley germination,Aleurone layer
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络