Two-dimensional mass spectrometry of biomolecules at the subfemtomole level
CURRENT OPINION IN CHEMICAL BIOLOGY(1998)
摘要
Multiple dimensions of unique molecular structure information can now be obtained from proteins and DNA using mass spectrometry. Less than 10(-16) mol of the active major histocompatibility complex signaling peptide in a mixture of thousands can be identified. For large proteins (>40 kDa), the high resolving power (>10(5)) and 10(-17) mol sensitivity of Fourier-transform mass spectrometry provide exact molecular weight values (+/- 1 or 2 Da) for mixture components, indicating errors or modifications compared with the predicted DNA sequence. Selecting a specific molecular species, its two-dimensional spectrum indicates the part of the molecule that is modified; a three-dimensional spectrum of that fragment further isolates the modification site.
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关键词
2D two-dimensional,3D three-dimensional,ESI electrospray ionization,FTMS Fourier-transform MS,LC liquid chromatography,MALDI matrix-assisted laser desorption/ionization,Mr relative molecular weight,MS mass spectrometry,MSn/MS/MS multidimensional MS,TOF time-of-flight
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