Proteotyping: A new approach studying influenza virus evolution at the protein level
Virologica Sinica(2007)
摘要
Phylogenetic methods have been widely used to detect the evolution of influenza viruses. However, previous phylogenetic studies
of influenza viruses do not make full use of the genetic information at the protein level and therefore cannot distinguish
the subtle differences among viral genes. Proteotyping is a new approach to study influenza virus evolution. It aimed at mining
the potential genetic information of the viral gene at the protein level by visualizing unique amino acid signatures (proteotypes).
Neuraminidase gene fragments of some H5N1 avian influenza viruses were used as an example to illustrate how the proteotyping
method worked. Bayesian analysis confirmed that the NA gene tree was mainly divided into three lineages. The NA proteotype
analysis further suggested there might be multiple proteotypes within these three lineages and even within single genotypes.
At the same time, some proteotypes might even involve more than one genotype. In particular, it also discovered some amino
acids of viruses of some genotypes might co-reassort. All these results proved this approach could provide additional information
in contrast to results from standard phylogenetic tree analysis.
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关键词
neuraminidase,avian influenza virus,genotype,h5n1,proteotyping,genetics,avian influenza,amino acid,bayesian analysis,phylogenetic tree
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