Plasma protein and apolipoprotein synthesis by human yolk sac carcinoma cells in vitro
In Vitro Cellular & Developmental Biology – Animal(1991)
摘要
Summary Three human yolk sac carcinoma cell lines were characterized for the expression of several markers. Each of the cell lines
expressed alpha-fetoprotein, without detectable levels of chorionic gonadotropin, and the level of alpha-fetoprotein expression
increased dramatically when the cultures were held without passage for extended periods. The secretion of a number of plasma
proteins was documented by metabolic labeling, immunoprecipitation, and gel analysis. The major plasma proteins detected were
alpha-1-antitrypsin, alpha-fetoprotein, transthyretin,β-2 microglobulin, and plasminogen, with lower levels of transferrin and complement C4 released. Apolipoproteins B, E, and
A1 were secreted in high levels as well and were found in the form of lipoprotein particles. Time course experiments on the
synthesis of apolipoproteins E and A1 indicated that, as with alpha-fetoprotein, the level of synthesis increased substantially
when the cultures were held without passage. The results indicate that these yolk sac carcinoma cells display a protein expression
profile similar to that observed for the human yolk sac, and the possibility that the cells may have the potential to differentiate
is discussed.
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关键词
yolk sac carcinoma,plasma protein synthesis,lipoprotein synthesis
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