Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR.

Morgan Guilbaud,Pierre de Coppet,Fabrice Bourion,Cinta Rachman,Hervé Prévost,Xavier Dousset

APPLIED AND ENVIRONMENTAL MICROBIOLOGY（2005）

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摘要

A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 X 10(2) CFU/cm(2).

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关键词

dna primers,quantitative risk assessment,biofilms,food industry,polymerase chain reaction,mortality rate,quantitative method,bacterial toxins,raw materials,real time pcr,heat shock proteins

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