Modell zur Analyse des knöchernen Anwachsverhaltens auf bioaktiv beschichteten Implantatoberflächen

Especially on the field of bone regeneration, transient and permanent implants are an important method of therapy in the Orthopaedic Surgery. In this context, bioactive surfaces on metallic implants provide an improved contact to the surrounding bone. The goal of our study was to establish an in-vitro test system to evaluate the on-growth of bone-derived cells on different surface coatings. Therefore, we invented a special kind of clamps made of commercially-pure (c-p) titanium and blasted with hydroxyapatite particles followed by electrochemically coating with calcium phosphate (BONIT (R)-HA, BONIT (R)). Definite pieces of human cancellous bone were attached to these clamps, inserted onto tissue culture plates and cultivated in DMEM for ten days. Finally, the contact area between human cancellous bone and the implant surface was analyzed and the spreading of osteoblast-like cells evaluated by scanning electron microscopy (SEM). A well-spread morphology of bone cells was observed on the implant surfaces coated with calcium phosphate (CaP). In comparison the clamps without CaP coatings showed only a marginal growth of bone cells on the clamp surface. The presented newly in-vitro test setup using titanium clamps coated with bioactive layers attached to human cancellous bone represents a well-functioning model for qualitative evaluation of bone on-growth.