Profiling the microRNAs

Profiling has contributed to the rapid growth of knowledge concerning microRNAs (miRNAs). The ability to measure all the known miRNAs by hybridization or by quantitative PCR has yielded insights into oncogenesis, stem differentiation, development, and disease. The steps toward discovery have often begun with profiling, followed by target prediction for miRNAs that are differentially expressed and then experimental validation of the targets Here we present some methodological detail on the profiling procedures used in the lab and their application.