My current work is focusing on how to break tolerance promoting the production of autoantibodies from B cells, particularly anergic B cells, by using conjugated vaccination. The immune system harbours large number of autoreactive B cells maintained anergy. Tolerance can be broken if sufficient stimulation signals are available, e.g. after immunisation with memory T cell recruiting conjugate vaccines. Recent work shows that anergic autoreactive B cells are competent to join germinal centre reaction and acquire mutations. Therefore, anergic B cells could be targets to induce response to tumour associated antigens. We recently developed a new genetically manipulated mouse model with hyperactive B cell receptor signalling. Most B cells in these mice are anergic, and we hope to utilize this new model to understand the phenotype of anergic B cells, and how to regulate anergic B cells to responding auto-antigens.