基本信息
浏览量:0
![](https://originalfileserver.aminer.cn/sys/aminer/icon/show-trajectory.png)
个人简介
Recombinational Repair of DNA
Repair of DNA by recombination is a high fidelity molecular system whose purpose is to restore genetic material lost as a result of breakage or other severe forms of damage to DNA molecules. The process relies on utilization of a homologous DNA sequence as a template to direct repair of the damaged molecule. The paradigm for recombinational repair holds that the broken molecule is resected from the end to reveal a protruding single strand which then pairs with the homologous sequence in the undamaged molecule forming a joint molecule with a displaced strand or D-loop. After some additional processing that includes a limited amount of DNA synthesis at the D-loop and that may include resolving a Holliday junction, the broken DNA is spliced back together with lost information restored.My laboratory uses a non-mainstream yeast, Ustilago maydis, for experimental work to study individual steps in the recombinational repair process. A key component in the pathway is a homolog of BRCA2, the product of one of the major breast cancer predisposition genes in human. It, in turn, is dependent on a small polypeptide, Dss1, that serves as an essential cofactor. We are trying to understand the function of the BRCA2 homolog in the recombination process and to understand the molecular mechanism of its regulation by Dss1. Our hope is that lessons learned from these basic investigations will bear on understanding how the more complex human BRCA2 protein operates as a tumor suppressor to maintain genomic stability.
Repair of DNA by recombination is a high fidelity molecular system whose purpose is to restore genetic material lost as a result of breakage or other severe forms of damage to DNA molecules. The process relies on utilization of a homologous DNA sequence as a template to direct repair of the damaged molecule. The paradigm for recombinational repair holds that the broken molecule is resected from the end to reveal a protruding single strand which then pairs with the homologous sequence in the undamaged molecule forming a joint molecule with a displaced strand or D-loop. After some additional processing that includes a limited amount of DNA synthesis at the D-loop and that may include resolving a Holliday junction, the broken DNA is spliced back together with lost information restored.My laboratory uses a non-mainstream yeast, Ustilago maydis, for experimental work to study individual steps in the recombinational repair process. A key component in the pathway is a homolog of BRCA2, the product of one of the major breast cancer predisposition genes in human. It, in turn, is dependent on a small polypeptide, Dss1, that serves as an essential cofactor. We are trying to understand the function of the BRCA2 homolog in the recombination process and to understand the molecular mechanism of its regulation by Dss1. Our hope is that lessons learned from these basic investigations will bear on understanding how the more complex human BRCA2 protein operates as a tumor suppressor to maintain genomic stability.
研究兴趣
论文共 7 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature (2010)
Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature (2009)
Natureno. 7026 (2005): 653-657
William K. Holloman,Richard L. Bennett, Allyson Cole-Strauss, David O. Ferguson,Kenan Onel, Mara H. Rendi, Michael L. Rice,Michael P. Thelen,Eric B. Kmiec
DNA Damage and Repairpp.539-556, (2003)
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn