Our laboratory studies macromolecular structures with an aim toward an in-depth understanding of structure-function relationships. X-ray diffraction analysis is our primary research tool, but we also employ other physical and biochemical methods of analysis. We strive to increase scientific reproducibility by developing methods and algorithms that help ensure structural models accurately reflect the macromolecules of interest and that scientific procedures and results can be reproduced. Most of the macromolecules that we study relate to one or more of a few broad biological areas: cellular signal transduction, metalloproteins, infectious diseases, antibiotic resistance, etc. We are currently members of the Center for Structural Genomics of Infectious Disease, an NIAID project focused on human pathogens. We use the proteins we study as test cases for methodology development. Our tools are used throughout the world and address a wide range of scientific problems, from diffraction image processing to metal environment geometries to various applications of big data concepts. We have developed many tools for biomedical researchers, including the HKL packages, CheckMyMetal, Molstack, and others that have garnered over 40,000 citations.