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职业迁徙
个人简介
My work in toxicology aims at linking results from test tube experiments with those of cellular studies. Analytical chemistry is very powerful for this purpose.
1. Photobiology. A large fraction of my activity deals with the formation of UV-induced photodamage in DNA. A main breakthrough was the development in the early 2000’s of a HPLC-mass spectrometry-based assay for the individual quantification of all possible photoproducts. This made possible the collection of novel data on the photochemistry of double-stranded DNA as well as major applications in mammalian cells. In particular, my work established that the UVA range of sunlight generates some of the photoproducts believed to be due only to UVB. I also gathered new information on DNA repair and worked with the cosmetics industry on photoprotection. In addition, I got involved in collaborative works in projects on microorganisms.
2. Formation of adducts by covalent binding of organic compounds to DNA. My first interest in this field was adducts of endogenous compounds like those produced by lipid peroxidation. More recently, I studied the formation of DNA adducts, in skin and in internal organs, in mice topically exposed to sulfur mustard. Currently, my main interest in chemical genotoxicity is the formation of DNA adducts to polycyclic aromatic hydrocarbons. I am in particular interested in identifying the factors modulating metabolism and genotoxicity of these compounds like mixture effects and co-exposure with sunlight. Assays are also available for oxidatively generated DNA lesions that could also be applied to works on oxidative stress like that induced by nanoparticles.
3. Development of analytical tools for the quantification of biomarkers. My most recent research topic is the development of HPLC-MS/MS methods for the quantification of specific molecular biomarkers of exposure in biological fluids. For example, we are working on the quantification of DNA and glutathione derivatives produced upon exposure to chemical warfare agents like sulfur mustard. The biomarkers are quantified in culture media, plasma and urine by a HPLC-MS/MS technique including isotopic dilution and on-line solid phase extraction.
研究兴趣
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Jouda Jakhlal,Clément Denhez, Stéphanie Coantic-Castex,Agathe Martinez,Dominique Harakat,Thierry Douki,Dominique Guillaume,Pascale Clivio
Organic & Biomolecular Chemistryno. 15 (2024): 3025-3034
Muriel Cario, Julie Scalia,Walid Mahfouf, Elodie Muzotte,Vincent Michaud,Claudio Plaisant,Jean-William Dupuy,Thierry Douki,Fanny Morice-Picard,Jérôme Rambert, Eric Perrier,Sandra Trompezinski,
Journal of Dermatological Science (2024)
Photochemistry and photobiology (2024)
Thierry Douki,Sylvain Caillat, Daniel Bacqueville, Camille Géniès, Celine Huyghe, Hélène Duplan,Jimmy Le Digabel, Christophe Lauze, Jerome Filiol, Razvan Marinescu, Karine Bouyer,Emmanuel Questel,
JID Innovationsno. 6 (2023): 100227-100227
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Thierry Douki,Sylvain Caillat,Daniel Bacqueville,Camille Géniès, C. Huyghe,Hélène Duplan,Jimmy Le Digabel,Christophe Lauze, Jérôme Filiol, R. Marinescu, Karine Bouyer,Emmanuel Questel,
JID Innovationsno. 6 (2023): 100227-100227
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DNA PHOTODAMAGE: From Light Absorption to Cellular Responses and Skin Cancer (2022): V-VI
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