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In order to obtain direct evidence of how the rotational mechanism operates so efficiently, it is essential to better understand the structure of the stator, the interactions of the rotor and stator with α3β3 and to learn how these interactions change during the catalytic cycle. Studies in our laboratory, supported by the Canadian Institutes of Health Research, are designed to provide this information so that the molecular mechanism of this extraordinarily important and complex enzyme may be understood. Our approaches include bioinformatics, site-directed mutagenesis, deletion analysis, chemical cross-linking, engineered disulfide bonds, fusion proteins, biophysical analysis, and collaborative high resolution structural analysis.
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