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In Saccharomyces cerevisiae most chitin is synthesized by Chs3p, which deposits chitin in the lateral cell wall and in the bud-neck region during cell division. We have recently found that addition of glucosamine (GlcN) to the growth medium leads to a three- to fourfold increase in cell wall chitin levels. We compared this result to the increases in cellular chitin levels associated with cell wall stress and with treatment of yeast with mating pheromone. Since all three phenomena lead to increases in precursors of chitin, we hypothesized that chitin synthesis is at least in part directly regulated by the size of this pool. This hypothesis was strengthened by our finding that addition of GlcN to the growth medium causes a rapid increase in chitin synthesis without any pronounced change in the expression of more than 6,000 genes monitored with Affymetrix gene expression chips. In other studies we found that the specific activity of Chs3p is higher in the total membrane fractions from cells grown in GlcN and from mutants with weakened cell walls.
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G. Guy Bushkin,Edwin Motari,Paula Magnelli,Marc-Jan Gubbels,Jitender P. Dubey, Katarzyna B. Miska,Esther Bullitt,Catherine E. Costello,Phillips W. Robbins,John Samuelson
mBiono. 5 (2012)
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GIARDIA: A MODEL ORGANISMpp.103-110, (2011)
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