基本信息
浏览量:5
职业迁徙
个人简介
The Laoratory of Dynamics of Macromolecular Assembly develops biophysical methods to study protein interactions and the assembly of multi-protein complexes. Hallmarks of multi-protein complexes are multi-valent interactions and cooperativity. In the molecular machinery of cellular processes these constitute ubiquitous mechanisms for the integration and transfer of information. Therefore, our focus is on the development of approaches for multi-component systems where several different macromolecular components interact to allow association and dissociation of different co-existing complexes in different states. We are interested in the characterization of the number of assembly states, and their size, shape, and the interaction energetics. Complementary to crystallographic techniques, such solution interaction studies can provide information on the assembly principles of structurally polymorph multi-protein complexes.
Our group has been particularly active in the development of quantitative hydrodynamic methods using sedimentation velocity analytical ultracentrifugation in conjunction with mathematical modeling of reaction/diffusion/sedimentation processes. We have recently embarked on the development of novel fluorescence detection approaches, which allow unprecedented sensitivity and resolution. We are also pursuing other techniques, including optical biosensing, isothermal titration calorimetry and global multi-method modeling approaches. We are dedicated to the dissemination of methodology, and have implemented our computational analysis methods in widely used software.
We are involved in several collaborative applications in various fields including immunological protein complexes, viral proteins, membrane receptor complexes, and eye lens crystallins.
研究兴趣
论文共 373 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
Journal of the American Chemical Societyno. 12 (2024): 8242-8259
Ai Nguyen,Huaying Zhao, Dulguun Myagmarsuren,Sanjana Srinivasan,Di Wu,Jiji Chen,Grzegorz Piszczek,Peter Schuck
biorxiv(2024)
Kristian Juul-Madsen,Peter Parbo,Rola Ismail,Peter L. Ovesen,Vanessa Schmidt,Lasse S. Madsen,Jacob Thyrsted, Sarah Gierl, Mihaela Breum,Agnete Larsen,Morten N. Andersen,Marina Romero-Ramos,
Huaying Zhao, Abdullah M Syed,Mir M Khalid,Ai Nguyen,Alison Ciling,Di Wu,Wai-Ming Yau,Sanjana Srinivasan, Dominic Esposito,Jennifer A Doudna,Grzegorz Piszczek,Melanie Ott,
MBIOno. 6 (2023): e0238823-e0238823
bioRxiv : the preprint server for biology (2023)
引用0浏览0WOS引用
0
0
Science Advancesno. 14 (2023)
加载更多
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn