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I am concerned with salt and water transport across cell membranes and whole epithelial tissues. These issues are essential to understanding regulation of salt and water balance by whole organisms. The laboratory has been studying how changes in intracellular parameters (such as calcium activity, pH, sodium activity, and free energy of hydrolysis of adenosine triphosphate (ATP)) might alter sodium transport. There is also interest in how biochemical modifications of the apical membranes (induced, for example, by phorbol esters) and biophysical alterations of the basolateral membranes might alter net sodium movement.
One major direction is to relate intracellular pH and calcium activity (measured with ion selective microelectrodes) to sodium permeability and intracellular sodium activity (measured with reference micropipets, while applying computer driven trains of voltage pulses). Concurrent studies are in progress ultilizing 31phosphorus and 19fluorine nuclear magnetic resonance (NMR) and patch clamping.
The major model system isthe isolated epithelium from frog skin, an analogue of the human distal nephron which can be studied by a variety of biophysical techniques. Collaborations are also being undertaken with the Glaucoma Unit to study fluid transport
across the ciliary body of the eye, and with the Renal Unit to study nephron tubules directly.
I am concerned with salt and water transport across cell membranes and whole epithelial tissues. These issues are essential to understanding regulation of salt and water balance by whole organisms. The laboratory has been studying how changes in intracellular parameters (such as calcium activity, pH, sodium activity, and free energy of hydrolysis of adenosine triphosphate (ATP)) might alter sodium transport. There is also interest in how biochemical modifications of the apical membranes (induced, for example, by phorbol esters) and biophysical alterations of the basolateral membranes might alter net sodium movement.
One major direction is to relate intracellular pH and calcium activity (measured with ion selective microelectrodes) to sodium permeability and intracellular sodium activity (measured with reference micropipets, while applying computer driven trains of voltage pulses). Concurrent studies are in progress ultilizing 31phosphorus and 19fluorine nuclear magnetic resonance (NMR) and patch clamping.
The major model system isthe isolated epithelium from frog skin, an analogue of the human distal nephron which can be studied by a variety of biophysical techniques. Collaborations are also being undertaken with the Glaucoma Unit to study fluid transport
across the ciliary body of the eye, and with the Renal Unit to study nephron tubules directly.
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Albert and Jakobiec's Principles and Practice of Ophthalmologypp.1-46, (2021)
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