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Since supramolecular ligands as well as certain proteins must ultimately be transported into cells, we will exploit large multishell CaP nanoparticles (100 nm) as transport vehicles across cell membranes. The pathway of the labeled cargo into and inside the cell can be monitored by confocal fluorescence microscopy. On the other hand, very small Au or SiO2 nanoparticles (2-5 nm) will be equipped with specific protein-binding peptides and receptor molecules provided by other partners from the CRC, which render the functionalized nanoparticles specific for central pores, flanks or interfaces of self-assembled protein machines.
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