Supra-subduction zone ophiolites such as the Troodos Ophiolite of Cyprus can give important information on the internal structure of the oceanic crust, and the processes by which it is formed. However, the origin of SSZ ophiolites is debated; they may form in a fore-arc location during subduction initiation; at a back-arc spreading centre immediately behind an active arc; at a plate ‚edge‘; or as a result of ridge subduction. Chemical analysis of ophiolite lavas, which could tell us how ophiolites were formed, is complicated by the extensive hydrothermal and seafloor alteration. Using microanalytical methods (electron microprobe, laser-ablation ICP-MS) at the GeoZentrum Nordbayern, we are able to obtain a complete major and trace element analysis of sub-mm sized chips of fresh volcanic glass from pillow lavas. We used these methods to examine the chemical stratigraphy of the Troodos Ophiolite in the Akaki Canyon section on the northern margin of the ophiolite. Akaki lavas have a wide range in trace element compositions – some have compositions similar to modern-day back-arc lavas, others extend towards the field of Troodos boninites, which were derived from a highly depleted, fluid-enriched mantle source that had subsequently been re-enriched by small degree melts. Based on these results we suggest that the Troodos crust was formed at a RTT or RTF triple junction, at a back-arc spreading centre that propagated into arc crust.