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职业迁徙
个人简介
In addition, in order to address the global shortage of donor tissues, were are currently involved in a study of corneal regeneration using tissue stem cells and induced pluripotent stem cells (iPS). In order to anatomically and functionally restore the cornea, our current studies involve the following: 1) Isolation of stem cells from the 3 layers of the cornea. 2) Induction of corneal cells from iPS cells. 3) Devising of tissue engineering techniques to produce 3-dimensional corneal tissue constructs. 4) Development of new surgical techniques. 5) Establishing the infrastructure required for the processing of safe tissue-engineered products.
Research activities
Isolation of mouse corneal epithelial stem cells
We devised a protocol using cholera toxin and serum-free medium to isolate corneal epithelial progenitor cells from a single mouse cornea (Ma X, Invest Ophthalmol Vis Sci 2009). One of the original cell lines has maintained a normal phenotype and karyotype through over 60 passages (Kawakita T. J Cell Mol Med. 2008).
Isolation of corneal stromal stem cells
We successfully isolated multipotent, neural crest-derived stem cells (COPs) from the adult murine cornea using the neurosphere technique (Yoshida S. Stem Cells 2006).
GMP grade cultivated epithelial sheets
We established a technique for engineering safe, transplantable epithelial sheets using donor human mesenchymal stem cells as feeder cells. The sheets are engineered in a cell processing center under strict sanitation standards. The initial clinical trial was approved by the Ministry of Health, Labour and Welfare.
Research activities
Isolation of mouse corneal epithelial stem cells
We devised a protocol using cholera toxin and serum-free medium to isolate corneal epithelial progenitor cells from a single mouse cornea (Ma X, Invest Ophthalmol Vis Sci 2009). One of the original cell lines has maintained a normal phenotype and karyotype through over 60 passages (Kawakita T. J Cell Mol Med. 2008).
Isolation of corneal stromal stem cells
We successfully isolated multipotent, neural crest-derived stem cells (COPs) from the adult murine cornea using the neurosphere technique (Yoshida S. Stem Cells 2006).
GMP grade cultivated epithelial sheets
We established a technique for engineering safe, transplantable epithelial sheets using donor human mesenchymal stem cells as feeder cells. The sheets are engineered in a cell processing center under strict sanitation standards. The initial clinical trial was approved by the Ministry of Health, Labour and Welfare.
研究兴趣
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Graefe's Archive for Clinical and Experimental Ophthalmologypp.1-9, (2024)
The Ocular Surface (2024): 139-144
Shintaro Yamaguchi,Junichiro Irie,Masanori Mitsuishi,Yuichi Uchino, Hideaki Nakaya,Ryo Takemura,Emi Inagaki, Shotaro Kosugi,Hideyuki Okano,Masato Yasui,Kazuo Tsubota,Kaori Hayashi,
ENDOCRINE JOURNALno. 2 (2024): 153-169
Ophthalmology Sciencepp.100491, (2024)
Junhan Chen,Shin-ichi Ikeda, Yajing Yang, Yan Zhang, Ziyan Ma, Yifan Liang,Kazuno Negishi,Kazuo Tsubota,Toshihide Kurihara
Molecular Medicineno. 1 (2024): 1-11
Longdan Kang,Shin-ichi Ikeda, Yajing Yang,Junhan Chen,Yan Zhang,Kazuno Negishi,Kazuo Tsubota,Toshihide Kurihara
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCEno. 8 (2023)
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bioRxiv (Cold Spring Harbor Laboratory) (2023)
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Yusaku Katada, Kazuho Yoshida, Naho Serizawa,Deokho Lee,Kenta Kobayashi,Kazuno Negishi,Hideyuki Okano,Hideki Kandori,Kazuo Tsubota,Toshihide Kurihara
iScienceno. 10 (2023): 107716-107716
Scientific Reportsno. 1 (2023): 1-9
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