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个人简介
Research Interests:
HIV, Influenza A virus
Biophysical, structural, and biochemical techniques including X-ray scattering, cryo-electron microscopy, hydrogen/deuterium-exchange mass spectrometry, and fluorescence microscopy to understand the function of viral machinery
Our lab uses complementary structural and biophysical methods to characterize the conformational changes viruses carry out in order to invade host cells. We focus on the transitions and the critical functional states that often are too transient or dynamic to characterize by classical structural methods. Understanding these events is essential to understanding mechanisms of cell entry, for understanding the activity of neutralizing antibodies, and also for developing novel antivirals that can target the virus prior to infection. Our core expertise is in using Hydrogen/Deuterium-Exchange Mass Spectrometry (HDX-MS), cryo-electron microscopy (cryo-EM) and small-angle X-ray scattering (SAXS) to monitor conformational change of protein complexes and viruses. An NIH K99/R00 Career Award supported my transition to studying influenza virus with techniques such as cryo-electron tomography and SAXS. Current projects apply cryo-ET, SAXS, and HDX-MS to dissect the spring-loaded membrane fusion mechanism of influenza virus hemagglutinin (HA) and HIV Env.
HIV, Influenza A virus
Biophysical, structural, and biochemical techniques including X-ray scattering, cryo-electron microscopy, hydrogen/deuterium-exchange mass spectrometry, and fluorescence microscopy to understand the function of viral machinery
Our lab uses complementary structural and biophysical methods to characterize the conformational changes viruses carry out in order to invade host cells. We focus on the transitions and the critical functional states that often are too transient or dynamic to characterize by classical structural methods. Understanding these events is essential to understanding mechanisms of cell entry, for understanding the activity of neutralizing antibodies, and also for developing novel antivirals that can target the virus prior to infection. Our core expertise is in using Hydrogen/Deuterium-Exchange Mass Spectrometry (HDX-MS), cryo-electron microscopy (cryo-EM) and small-angle X-ray scattering (SAXS) to monitor conformational change of protein complexes and viruses. An NIH K99/R00 Career Award supported my transition to studying influenza virus with techniques such as cryo-electron tomography and SAXS. Current projects apply cryo-ET, SAXS, and HDX-MS to dissect the spring-loaded membrane fusion mechanism of influenza virus hemagglutinin (HA) and HIV Env.
研究兴趣
论文共 123 篇作者统计合作学者相似作者
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ACS infectious diseasesno. 6 (2023): 1180-1189
bioRxiv (Cold Spring Harbor Laboratory) (2023)
Nicolas Goldbach, Issa Benna,Basile I. M. Wicky,Jacob T. Croft,Lauren Carter,Asim K. Bera,Hannah Nguyen,Alex Kang,Banumathi Sankaran,Erin C. Yang,Kelly K. Lee,David Baker
Protein Scienceno. 11 (2023)
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The Journal of biological chemistryno. 6 (2023): 104765-104765
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bioRxiv : the preprint server for biology (2023)
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CELL REPORTSno. 12 (2023): 113553-113553
npj Vaccinesno. 1 (2023): 190-190
Nicolas Goldbach, Issa Benna,Basile I. M. Wicky,Jacob T. Croft,Lauren Carter,Asim K. Bera,Hannah Nguyen,Alex Kang,Banumathi Sankaran,Erin C. Yang,Kelly K. Lee,David Baker
PROTEIN SCIENCEno. 11 (2023)
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