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The Schwender group is interested in increasing the basic understanding of control and regulation of plant central metabolism, with focus on heterotrophic sink tissues like seeds. We use isotope labeling experiments, mathematical models and computer simulation to describe and analyze metabolism quantitatively. In particular we have extensive experience in steady-state stable isotope labeling to determine the flux distribution in central carbon metabolism. Cultures of developing embryos of Brassica napus and other brassicaceae species turned out to be excellent in vitro models to study seed development and storage synthesis. Growing in culture, embryos are labeled with a variety of 13C-labeled precursors and individual C-atoms are traced through the metabolic network by analyzing the label in metabolites and end products by GC/MS and NMR. Different nutritional conditions or genotypes can be analyzed and compared.
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GLOBAL CHANGE BIOLOGY BIOENERGYno. 7 (2023): 840-866
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NEW PHYTOLOGISTno. 5 (2023): 1539-1541
New Phytologistno. 2 (2023): 724-736
FRONTIERS IN PLANT SCIENCE (2022): 955589
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