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How the pluripotent lineage is established during development is also an interesting question, which we address using molecular and genetic approaches incorporating mouse lines with specific and inducible deletions in key pluripotency factors. We quantify transcripts using single cell technology and proteins by quantitative immunofluorescence. Conditional deletion mutants provide a valuable system with which to explore the temporal requirement for factors of interest during entry and exit from pluripotency and lineage priming. ES cells maintain a molecular profile closely related to the early epiblast from which they originate. They also retain the capacity to integrate into preimplantation embryos. We exploit this phenomenon to challenge the host embryo to react to the influx of epiblast cells and monitor its response in terms of selective elimination or modification of lineage decisions. Microinjection, live imaging and immunohistochemistry comprise the main tools for this project.
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biorxiv(2024)
V. Gonzalo-Nadal, A. Kohl,M. Rocchi, B. Brennan, J. Hughes,J. Nichols, A. Da Silva Filipe, J. I. Dunlop, M. Fares, J. J. Clark, R. Tandavanitj, A. H. Patel,
JOURNAL OF SMALL ANIMAL PRACTICEno. 2 (2024): 132-143
Takuya Azami, Bart Theeuwes, Mai-Lihn Ton,William Mansfield,Masaki Kinoshita,Berthold Göttgens,Jennifer Nichols
crossref(2024)
Current Opinion in Genetics & Development (2023): 102130-102130
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bioRxiv (Cold Spring Harbor Laboratory) (2023)
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Nature Cell Biologyno. 7 (2023): 1061-1072
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biorxiv(2023)
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