I am interested in protein-membrane interactions. We are studying how proteins interact with lipid membranes at the molecular level. Specifically, we study pore forming proteins that are able to perforate lipid membranes in a multi-step process involving membrane binding, oligomerization and pore formation. We study important regulators of immune system (perforin), bacterial virulence factors (listeriolysin), Nep1-like proteins and proteins similar to toxins from sea anemones (actinoporin-like proteins). We employ various biochemical and biophysical approaches with an emphasis on surface plasmon resonance, which allows studying molecular interactions. We are developing and improving protocols for the study of protein binding to lipid membranes.