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Research in this laboratory focuses on the biosynthesis and actions of estradiol. In tissues where aromatase (cytochrome P450 aromatase, estrogen synthetase) and estrogen receptors (ER) are colocalized, estradiol has discrete, local actions (paracrine, autocrine) that differ qualitatively and quantitatively from those exerted by the circulating hormone (endocrine). We are investigating the structure, function, regulation, and evolution of genes encoding aromatase (Cyp19) and ER (Esr) in neural tissues and the physiological and developmental consequences of estrogen formation in specific cells and circuits of the brain and retina. A component of the project is to define the role of different ER genes and splice variants in mediating estrogen actions in different tissue types and developmental stages; determine mechanisms by which estrogen-like environmental chemicals disrupt normal endocrine and neuroendocrine processes of physiology and development; and identify physiological, genetic, and epigenetic adaptations in estrogen signaling pathways that are driven by long term, multigenerational exposure of organisms to estrogenic environments. We use a wide range of animal models from fish to mammals and rely primarily on methods of cell biology (tissue culture, light and electron microscopy, image analysis), protein and steroid biochemistry (enzymology, SDS-PAGE and immunoblot analysis), and molecular biology (cDNA and gene cloning and PCR-based methods of RNA & DNA analysis).
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Karen G Burnett A,Lisa J Bain,William S Baldwin,Gloria V Callard,Richard T Di Giulio E, David H Evans F, Marta Gomezchiarri G, M H, Cindi A Hoover C, S H, Fumi Katoh J, Deborah L Maclatchy I,
mag(2013)
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G.V. Callard,A.M. Tarrant,A. Novillo, P. Yacci, L. Ciaccia,S. Vajda,G.-Y. Chuang,D. Kozakov, S.R. Greytak, S. Sawyer, C. Hoover,K.A. Cotter
mag(2008)
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