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Our current focus is on molecular mechanisms of protein and lipid trafficking between the endoplasmic reticulum (ER) and the Golgi complex in. Several of the genes involved in this process have been identified and their function may be assessed in cell-free assays that reproduce transport. Through a combined genetic and biochemical approach, the mechanisms underlying secretory cargo selection and vesicle budding from the ER as well as vesicle targeting and fusion with the Golgi complex are under study. This intracellular transport step is essential for cellular function and our studies are basic for understanding numerous health related issues including cholesterol regulation, cystic fibrosis and diabetes.
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